The group's charter required that the day of arrival be observed yearly as a "day of thanksgiving" to God. On that first day, Captain John Woodlief held the service of thanksgiving. As quoted from the section of the Charter of Berkeley Hundred specifying the thanksgiving service: "We ordaine that the day of our ships arrival at the place assigned for plantacon in the land of Virginia shall be yearly and perpetually kept holy as a day of thanksgiving to Almighty God."

 

During the Indian massacre of 1622, nine of the settlers at Berkeley Hundreds were killed, as well as about a third of the entire population of the Virginia Colony. The Berkeley Hundred site and other outlying locations were abandoned as the colonists withdrew to Jamestown and other more secure points.

After several years, the site became Berkeley Plantation, and was long the traditional home of the Harrison family, one of the First Families of Virginia. In 1634, it became part of the first eight shires of Virginia, as Charles City County, one of the oldest in the United States, and is located along Virginia State Route 5, 『which runs parallel(平行) to the river's northern borders past sites of many of the James River plantations between the colonial capital city of Williamsburg (now the site of Colonial Williamsburg) and the capital of the Commonwealth of Virginia at Richmond.』